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2 edition of Investigation of factors influencing radiation resistance of Micrococcus radiodurans found in the catalog.

Investigation of factors influencing radiation resistance of Micrococcus radiodurans

Jong Sun Lee

Investigation of factors influencing radiation resistance of Micrococcus radiodurans

  • 108 Want to read
  • 3 Currently reading

Published .
Written in English

    Subjects:
  • Radiobiology.

  • Edition Notes

    Statementby Jong Sun Lee.
    The Physical Object
    Pagination91 leaves, bound :
    Number of Pages91
    ID Numbers
    Open LibraryOL14332753M

    Biology of Extreme Radiation Resistance: The Way of Deinococcus radiodurans Anita Krisko1 and Miroslav Radman1,2 1Mediterranean Institute for Life Sciences, Split, Croatia 2Faculte ´de Medecine, Universite Rene Descartes—Paris V, INSERM U, Paris, France Correspondence: @ D. radiodurans is a polyextremophile (48), showing remark-able resistance to a range of severe damage caused by ionizing radiation, desiccation, UV radiation, oxidizing agents, or elec-trophilic mutagens (40). This bacterium is most famous for its resistance to ionizing radiation. Objective: the present studies discuss impact of two different kinds of irradiations (high energy Nd-Laser and Gamma – irradiations which has oxidative nature by additional to its thermal effects) on the micro-structural parameters (grains,particles sizes) of milk samples. Methodology: the investigated samples will be examined spectrophotometrically and structurally before and after.


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Investigation of factors influencing radiation resistance of Micrococcus radiodurans by Jong Sun Lee Download PDF EPUB FB2

Graduate Thesis Or Dissertation Investigation of factors influencing radiation resistance of Micrococcus radiodurans Public Deposited. Analytics × Add to Author: Jong Sun Lee. BIOCHIMICA ET BIOPHYSICA ACTA BBA 9S THE RESISTANCE OF MICROCOCCUS RADIODURANS TO ULTRAVIOLET RADIATION I.

ULTRAVIOLET-INDUCED LESIONS IN TIrE CELL'S DNA JANE K. SETLOW ANn D. DUGGAN* Biology Division, Oak Ridge National Laboratory**, Oak Ridge, Tenn. (U.S.A.) (Received January 28th, ) SUMMARY Micrococcus radiodurans seems Cited by: Deinococcus radiodurans is an extremophilic bacterium, one of the most radiation-resistant organisms known.

It can survive cold, dehydration, vacuum, and acid, and is therefore known as a polyextremophile and has been listed as the world's toughest bacterium in Class: Deinococci. A factor responsible for radiation resistance has been extracted from the cells of urans; this factor in low doses sensitizes and in high doses protects Escherichia coli B/r from the.

Deinococcus radiodurans is well known for its extraordinary resistance to radiation and desiccation. It was identified that ionizing radiation sensitive mutants are more vulnerable to desiccation (Mattimore and Battista, ), indicating that these effects trigger similar stress response mechanisms in the by: 3.

Abstract. Micrococcus radiodurans, a gram-positive, nonsporing, red-pigmented bacterium, is the type species of a small group of bacteria, the members of which are characterized by extreme resistance to both the lethal and mutagenic effects of ionizing and ultraviolet (UV) show no loss of viability up to doses of krad or Jm-2 of ionizing or UV radiation, respectively Cited by: Micrococcus radiodurans strains R I and R II5 were tested for susceptibility to several antibiotics by the standardized Bauer-Kirby disc diffusion and tube dilution techniques, using different size of inoculum irradiated at doses of, and krad; a control without by: 2.

Out of these, D. radiodurans is the most extreme in terms of radiation resistance and desiccation tolerance. Cyanobacteria such as Chroococcidiopsis and fungi such as Filobasidiales also have the characteristic of radiation resistance but not to the extent of D.

radiodurans. To explore the radiation-resistance mechanisms in bacteria, a radiation-resistant strain SC was isolated from the surrounding area of a 60Co-γ radiation facility. SC could survive up to 8 kGy dose of gamma irradiation and was identified as Micrococcus luteus by phylogenetic analysis of 16S rRNA gene by: 3.

Ionizing Radiation Resistance in Deinococcus Radiodurans the maximum tolerated dose of ionizing radiation is 50 kGy in urans (Ghosal et al., ). The extreme radiation resistance of this bacterium is due to many factors (Figure 1).

DNA Repair Mechanisms DSBs are the most lethal damage caused by ionizing radiation. The influence of some factors involved in radappertization of meats on the radiation resistance of two isolates of Moraxella-Acinetobacter (M-A), Microcodes radiodurans, Escherichia coli, and.

Description and significance. Deinococcus radiodurans was first discovered in in a can of ground meat that had been treated with large doses of radiation to remove all hazardous bacteria from the product. Since then this species has been intensely studied for its radiation resistant properties.

It has been known to withstand radiation levels of up to 1, times that which would kill a. The addition of NZ-case (a tryptic digest of casein) to a growth medium (PC) consisting of tryptone, glucose, and yeast extract caused a significant decrease in γ radiation resistance of Micrococcus radiodurans.

The level of radiation resistance was inversely related to the concentration of by:   As shown in Fig. 1, Deinococcus has a systematic radiation-resistance mechanism. Ionizing radiation (IR) can produce reactive oxygen species (ROS) and cause double-stranded DNA breaks (DSBs).

Because of the DNA protection mechanism, the genomes of D. radiodurans are tightly linked and form ring-like nuclei under IR. The level of DNA damage in D. radiodurans and E. coli Cited by: 1. Deinococcus radiodurans is a robust bacterium best known for its capacity to repair massive DNA damage efficiently and accurately.

It is extremely resistant to many DNA-damaging agents, including ionizing radiation and UV radiation ( to nm), desiccation, and mitomycin C, which induce oxidative damage not only to DNA but also to all cellular macromolecules via the production of Cited by: The ionizing-radiation resistance of.

radiodurans. has been considered to be difficult to explain from an evolutionary point of view. Battista and his colleague [3] argued that the radiation resistance of. radiodurans.

might be a fortuitous consequence of an evolutionary process that permitted this bacterium to. A total of 36 microorganisms, comprising 19 species of 11 genera, were screened for radiation resistance with 60Co gamma rays at a radiation temperatore of ± 2 C in phosphate buffer (pH ) under vacuum.

Micrococcus radiodurans was the most resistant by:   Recent investigations report on biofilm formation of a minor genetically modified D. radiodurans str although these investigation requires further critical : Emanuel Ott, Felix M.

Fuchs, Ralf Moeller, Ruth Hemmersbach, Yuko Kawaguchi, Akihiko Yamagishi, Wolf. AN ABSTRACT OF THE THESIS OF Kenneth Louis Krabbenhoft for the Ph. D, in Microbiology (Name) (Degree) (Major) Date thesis is presented T- Title SIGNIFICANCE OF PIGMENTS IN RADIATION RESISTANCE OF MICROCOCCUS RADIODURANS Abstract approved (Major professor) An ecological study of a microorganism similar to that iso- lated in and designated as Micrococcus radiodurans.

Age-dependent fluctuation of radiation resistance of Micrococcus radiodurans was investigated. The fluctuation observed in cells grown in synthetic media was less than 10% during 44 hrs of growth. The cells harvested 9 hrs after inoculation were the most radiation resistant. The wet mass and DNA contents of these cells were low.

S-layers are crystalline arrays formed by proteinaceous subunits that cover the outer surface of many different kinds of microorganisms. This “proteinaceous cover” is particularly important in the case of ionizing-radiation-resistant bacteria (IRRB) that might be used in bioremediating hazardous and radioactive wastes (HRW).

Despite the exponential growth in the number of comparative Cited by: 2. Deinococcus radiodurans (D. radiodurans) is a Gram-positive, pink-pigmented, high G + C bacterium that belongs to the Deinococcus–Thermus was isolated from gamma-radiated canned meat and is best known for its extraordinary resistance to ionizing irradiation [1,2,3].However, previous studies also reported that this bacterium presents a rapid response and adaptation to a wide variety Author: Dong Xue, Yun Chen, Jiang Li, Jiahui Han, Zhengfu Zhou, Wei Zhang, Ming Chen, Min Lin, Marc Ongena.

Characteristics of Radiation-Resistant Micrococcus, Mechanism of Microbial Radiation Resistance, Factors Affecting Radiation Resistance, Article Background Mutational Features of the Radiation-Resistant Bacterium Deinococcus radiodurans Hongan Long,*,y,1 Sibel Kucukyildirim,y,2 Way Sung,1 Emily Williams,1 Heewook Lee,3 Matthew Ackerman,1 Thomas G.

Doak,1,4 Haixu Tang,3 and Michael Lynch1 1Department of Biology, Indiana University, Bloomington 2Department of Biology, Hacettepe University, Ankara, Turkey. Extreme radiation and oxidative stress resistance in Deinococcus involves multiple factors and well-regulated mechanisms.

Deinococcus radiodurans was the first species of the genus Deinococcus that was isolated, and was also the first Deinococcus species for which the genome sequence was thoroughly analysed (White et al.

; Makarova et al Cited by: Understanding Oxidative Stress Response in Deinococcus Radiodurans Deinococcus Radiodurans. Also referred to as “Conan the Bacterium” D.

Radiodurans belongs to a small family of extremophiles that share an uncanny ability to survive extreme amounts of surprisingly, D. Radiodurans was first isolated in from a canned meat product that had been treated with gamma radiation.

Deinococcus wulumuqiensis R12 is a red-pigmented extremophilic microorganism with powerful antioxidant properties that was isolated from radiation-contaminated soil in Xinjiang Uyghur Autonomous Region of China. The key carotenoid biosynthesis genes, crtE, crtB and crtI, which are related to the cells’ antioxidant defense, were identified in the sequenced genome of R12 and by: 3.

Low-Temperature Ionizing Radiation Resistance of Deinococcus radiodurans and Antarctic Dry Valley Bacteria Lewis R. Dartnell,1,2 Stephanie J. Hunter,3 Keith V. Lovell,4 Andrew J. Coates,5 and John M.

Ward3 Abstract The high flux of cosmic rays onto the unshielded surface of Mars poses a significant hazard to the survival ofCited by:   Factors influencing radiation resistance. The extent of D.

radiodurans resistance to ionizing radiation depends strongly on physiological conditions, such as the age of the culture, the cell concentration, the growth medium, the pH, the irradiation medium, the.

@article{osti_, title = {UV light-induced survival response in a highly radiation-resistant isolate of the Moraxella-acinetobacter group}, author = {Keller, L.C. and Thompson, T.L. and Maxcy, R.B.}, abstractNote = {A highly radiation-resistant member of the Moraxella-Acinetobacter group, isolate 4, obtained from meat, was studied to determine the effect of preexposure to UV radiation on.

Metal Balance Helps Explain Survival of Microbial "Superhero" by Marcia Stone. Microbe, 4, () Bacteria belonging to the family Deinococcaceae are some of the most radiation-resistant organisms yet discovered.

Deinococcus (Micrococcus) radiodurans strain R1 (ATCC BAA) was first reported in by A. Anderson and coworkers of the Oregon Agricultural Experimental. Radioresistance is the level of ionizing radiation that organisms are able to withstand. Ionizing-radiation-resistant organisms (IRRO) were defined as organisms for which the dose of acute ionizing radiation (IR) required to achieve 90% reduction (D10) is greater than gray (Gy) Radioresistance is surprisingly high in many organisms, in contrast to previously held views.

Deinococcus radiodurans is well known for its unusual resistance to different environmental stresses. Recently, we have described a novel complex composed of the surface (S)-layer protein DR_ and the carotenoid deinoxanthin.

We also showed a role of this complex in the UV resistance under desicc. One bacterium best known for its extreme resistance to the lethal effects of ionizing radiation is the aerobic gram-positive Deinococcus radiodurans.

Two studies focused on the transcriptome of D. radiodurans, using different physiological growth states (exponential vs. stationary phase) and different doses of γ radiation (3 kGy vs. 15 kGy Cited by: Subfreezing temperatures raise the radiation resistance of vegetative cells as the water e.g.

Deinococcus radiodurans, formerly known as Micrococcus radiodurans, which behaves like a Gram-negative bacterium. They possess Lund BM ().

Quantification of factors affecting the probability of development of pathogenic bacteria, File Size: KB. Abstract. The bacterium Deinococcus radiodurans is a champion of extreme radiation resistance that is accounted for by a highly efficient protection against proteome, but not genome, damage.

A well-protected functional proteome ensures cell recovery from extensive radiation damage to other cellular constituents by molecular repair and turnover processes, including an efficient repair of Cited by:   Sustainable life support on Mars – the potential roles of cyanobacteria - Volume 15 Special Issue - Cyprien Verseux, Mickael Baqué, Kirsi Lehto, Cited by: We have recently shown that Deinococcus radiodurans and other radiation resistant bacteria accumulate exceptionally high intracellular manganese and low iron levels.

In comparison, the dissimilatory metal‐reducing. Fifty years ago, scientists discovered D. radiodurans, leading to speculation that the incredible degree of resistance exhibited by the bacteria has to. Members of the widespread IS/IS bacterial insertion sequence (IS) family transpose using obligatory single-strand (ss) DNA intermediates.

This distinguishes them from classical IS, which move via double-strand (ds) DNA intermediates (see Siguier et al., this volume). Members of this family also differ fundamentally from classic IS in their by:.

Deinococcus radiodurans: Revising the Molecular Basis for Radiation Effects on Cells radiodurans (> Fig. ), first isolated from irradiated meat in by Arthur (Andy).Factors affecting transposition activity of IS50 and Tn5 ends.

Gene – Multiplicity of genome equivalents in the radiation-resistant bacterium Micrococcus radiodurans. J Bacteriol 71– Harsojo, Kitayama S, Matsuyama A. Genome multiplicity and radiation resistance in Micrococcus radiodurans.

J Biochem Cited by: Ultraviolet Radiation Resistance as The Rule For HAAL'S Microbes. Ultraviolet radiation is part of the solar electromagnetic spectrum, and it is an essential factor for many global biological and environmental phenomena. There are three major subtypes of UV rays, namely, UV-A (– nm), UV-B (– nm) and UV-C (– nm).